关键词: 烟雾染毒, 存活率, 氧浓度

Abstract: Objective: To investigate the effect of the modified method of smoke exposure on the survival rate of mice. Methods: on the basis of the common smoke exposure method, a device for increasing the oxygen quantity and increasing the oxygen amount at the bottom of the contaminated tank and monitoring the temperature simultaneously was made. The object of Kunming mice were divided into control group (not exposed in the common exposure device, exposure group (1), normal exposure method, exposure group (2) were improved) experiment, analyzing the application of spss20.0 statistical software were compared between the survival rate of mice, with oxygen concentration toxic unit and the changes of total the concentration of suspended particles. Results: a total of 160 Kunming mice were selected and randomly divided into 40 groups, 60 rats exposed to 1 groups, 2 rats exposed to 60 groups, both sexes were half and half. The experimental results showed that the survival rate in 2 groups of mice was significantly higher than that in group 1, the difference was statistically significant (x2=7.647 P=0.02<0.05), the oxygen concentration in the 1 groups were significantly lower than the exposure device of oxygen concentration of 2 groups, 2 groups of oxygen concentration fluctuations in the exposure and the device is small, the difference was statistically significant (F=5.260, p=0.045<0.05). There was no statistical difference in the concentration of TSP between the 1 groups and the 2 groups, indicating that the improved smoke exposure device did not affect the dose and exposure process of the toxicant. Conclusion: the improved smoke exposure device will reduce the mortality of the experimental animals under non pharmacological factors, and does not affect the experimental exposure process.

Key words: smoke exposure, survival rate, oxygen concentration