关键词: 抑郁症模型, 长期精神紧张, C57BL/6 J小鼠

Abstract: Objective Depression model was established with long-term mental stress stimulation. Methods 14 male C57BL/6J mice aged 4 weeks (body weight 11.05 1.65 g) were randomly divided into control group and LTMS group. In the LTMS group, the cats were kept in a single cage for 21 consecutive days and were given shock for 3h and sleep deprivation for 24h.The experimental group judged whether the modeling was successful or not by measuring the change of food intake, the overhead cross maze experiment, the open field experiment and the change of forced swimming behavior. Results Compared with the control group, the food intake of LTMS group was significantly reduced (p<0.01), and the forced swimming time was significantly prolonged (p<0.01).In the open-field experiment, the number of traversal and the number of upright position of LTMS group mice were significantly less than that of the control group, and the difference was extremely significant (p<0.01).In the overhead cross maze experiment, the residence time of the closed arm of LTMS group mice was significantly shortened, and the difference was extremely significant (p<0.01), and the proportion of the times of entering the open arm was reduced, and the difference was significant (p<0.05). Conclusions The long-term mental stress model can be used as the depression model because it shows the similar symptoms of behavior despair, decreased activity ability, loss of interest, anxiety and clinical manifestations of depression.

Key words: Depression model, Chronic mental stress, C57BL / 6 j mice