关键词: 小鼠, 人工喂养, 母乳喂养

Abstract: Objective This study aimed at developing a new experimental model for the artifically rearing of neonatal mice to lay the foundation for further study of the nutritional needs and growth of mice. Method Five pregnant BALB/c mice of 10—12 gestation days were kept until deliver. Total 27 neonatal mice were immediately randomized into two groups at postnatal day 3, one for maternal rearing and another for artificial rearing to postnatal day 14. Result 100% of the artificial rearing mice survived at 10 days, 92.9% at 11 days, 78.6% at 12 days, 50% and 35.7% at 13 days and 14 days, respectively. However, significant differences of body weight were found between the maternal reared mice and the artificial reared mice at postanal day 10 (3.02±0.67 vs. 9.00±0.70，P<0.01). Conclusion The new formula and nipple at least succesfully achieved the artifical rearing of neonatal mice to some extent, however, further study was needed to improve the survival rate and increase the body mass of artificially fed mice during lactation.

Key words: mice, artificial rearing, maternal rearing