关键词: 马立克氏病病毒, 液相芯片, 检测

Abstract: Objective In order to establish a double-antibody sandwich liquid chip technology assay for detection of Marek’s Disease Virus. Method The capture antibody was coupled to the 19 microsphere. We optimized the coupling capacity of capture antibodies, the working concentration of detection antibody and SA-PE, respectively. The specificity, sensitivity, reproducibility and clinical samples of this method were evaluated. Result The optimal coupling capacity of capture antibody was 2 μg of 1×106microspheres. The optimal working concentration of detection antibody and SA-PE was 2 μg/mL and 4 μg/mL, respectively. The method was specific for Marek’s Disease Virus, and there was not cross reaction with other viruses; The limit of detection reached 125 pfu/mL; Reproducibility analysis showed that the intra- and inter-assay coefficient of variation was 0.85% and 2.4%, respectively; The detection rate of 58 clinical samples was 31%. Compared with PCR, the total coincidence rate was 94.8%. Conclusion These result showed that the method was high specificity, sensitivity and reproducibility. The assay has important significance for monitoring, preventing and controlling Marek’s Disease, and could be useful for screening of specific-pathogen-free (SPF) chicken.

Key words: Marek’s Disease Virus, liquid chip, detection