关键词: 稀有鮈鲫组织形态学被, 呼吸系统, 心血管系统

Abstract: Abstract: Objective The research is concerned primarily with genomics, proteomics and hormone so far in ecology, toxicology, parasites and aquaculture. In this paper, the histological description was given by systematically histological observation of normal male and female Gobiocypris rarus, which provides a more detailed background and theoretical basis of this experimental animal. Method Gobiocypris rarus in the experiment was provide by Insitute of Hydrobiology (CAS). The fish was fixed in Bouin`s fluid for 24-48 hours and then washed with running water slightly, and put into decalcifying fluid. Then each fish was cut into several small pieces. The samples were dehydrated with alcohol and terpilenol. The specimen in solid paraffin was cut into serial slices with thickness of 3 μm. According to the routine protocol, the slices were dyed by Hematoxylin and Eosin (H&E) staining. Transverse and sagittal section pictures of the fish were collected by a optical microscope. Result Gobiocypris rarus, a member of the Gobioninae subfamily, belongs to the Osteichthyes. The skin of Gobiocypris rarus is composed of an outer epidermis and a underlying dermis, which forms the external protection of body against mechanical injury and noxious agents. Scales can be seen on the surface of the skin, which means Gobiocypris rarus using its gills, rathan than the skin, to breathe. In Gobiocypris rarus, like in other non-mammalian vertebrates, erythrocytes are oval in shape and always contain a nucleus, which spread in heart, arteries, veins and vessels. Under microscope, the sinus venosus, the atrium, the ventricle and the highly elastic bulbus arteriosus, these four chambers compose heart. Conclusion As a specific fish in China, the skin and the organs of respiratory and cardiovascular systems in Gobiocypris rarus were systemly observed and decribed in histology. The result realibly offers a theoretical basis to experiments with the animal.

Key words: Gobiocypris rarus, histomorphology , respiratory system, cardiovascular system