关键词: <, p>, 稀有鮈鲫, 配合饲料, 生长, 繁殖, 饲养策略<, /p>

Abstract: Abstract: Objective To explore applicable artificial diets and its suitable rearing strategies for this laboratory fish ( Gobiocypris rarus) and improve its nutrition standardization． Method Artificial diets were designed based on nutrition requirement and tested in growth and reproduction experiments，and effects of different rearing strategies such as temperature，feeding frequency and stocking density on the growth and reproduction were revealed fed with suitable artificial diets． Ｒesult Diets with 25% ～ 40% crude protein could meet the requirement for fast growth of rare minnow，especially the diet with 30% crude protein，6% crude lipid and 12. 5 kJ/g gross energy，could also promote the reproductive performance，and the success rate of reproduction could achieve 69. 4% compared to those fed with red worms ( Chironomus larva) ． The spawning interval decreased with increasing dietary protein． The growth rate increased with increasing water temperature in 24 ～ 28℃，but descended with over high temperature． The optimal culture temperature was 24℃ when feeding 1 ～ 3 meals a day． The growth rate decreased with increased stocking density，the feed efficiency was higher in 0. 8 fish /L and the optimal stocking density was 1. 6 fish /L． Conclusion The artificial diets with 30% crude protein，6% crude lipid and 12. 5 kJ/g gross energy could meet the requirement for fast growth of rare minnow，also，the parent fish could reproduce regularly． Too high or too low temperature could affect the growth of rare minnow adversely． High feeding frequency could not always promote the growth and feed conversion rate． The optimal culture temperature for rare minnow was 24℃ to 26℃，and feeding frequency was 2 meals per day． Stocking density obviously affected the growth and gonad development of rare minnow，the optimal density in recirculating aquaculture system should be controlled at 1. 6 fish /L or lower．

Key words: <p>rare minnow, artificial diets, growth, reproduction, rearing strategies</p>