关键词: <, p>, 大鼠仙台病毒, 能力验证, 酶联免疫吸附试验, 免疫荧光实验<, /p>

Abstract: Abstract: Objective To investigate the technical level of rat Sendai virus antibody detection for lab animal testing laboratories． To explore and solve the existing problems in Sendai virus detection，so as to promote quality management of laboratories and improve the detection level． Method To prepare rat serum samples after calibration and test their stability and homogeneity according to CNAS． The samples were numbered randomly and distributed to the participating laboratories． Each laboratory received three samples and must send results to us in a specified time． The results were expressed as positive or negative． If the test results were consistent with expected results，the laboratory was judged satisfactory． If the results were not entirely consistent or feedback was not got on schedule，the laboratory was judged unsatisfactory． Ｒesult Twenty-eight laboratories from 18 provinces enrolled in the program and sent the results in a specified time． The results of 25 laboratories were all satisfactory，which were 89. 3% of all laboratories． 3 laboratories were not satisfactory，one of which has one sample which result did not match and the other 2 laboratories have 2 samples which did not match． 27 of 28 laboratories adopted ELISA and one lab used IFA． The original records were authentic but individual labortories have some problems，such as reagent is not clear，operation process is not detailed and positive results were not retested． Conclusion The Sendai virus detection ability level of animal test laboratories is high． The implementation of proficiency testing can reflect the detection level of laboratories．

Key words: <p>Ｒat sendai virus, proficiency testing, ELISA, Immunofluorescence assay</p>