关键词: <, p>, p53 + / － 基因敲除小鼠, 脏器质量, 血液学指标, 血生化指标<, /p>

Abstract: Abstract: Objective To determine body weight，organ weight，hematological and blood biochemical parameters of p53 + / － gene knockout mice，constructed by us and named as B6 － Trp53tm1 /NIFDC，after MNU administration of MNU and to provide background data for candidate animal models used as short-term in vivo alternative carcinogenicity study in the field of preclinical safety evaluation of drugs． Method There were four different groups，negative control group: wild type C57BL /6 mice treated with saline solution; vehicle control group: B6 －Trp53tm1 /NIFDC mice treated with citrate buffer solution; MNU group 1: B6 － Trp53tm1 /NIFDC mice treated with 75 mg /kg MNU and MNU group 2: wild type C57BL /6 mice treated with 75mg /kg MNU． Both negative control group and MNU group 2 have 20 wild type animals，10 male 10 female． Both vehicle control group and MNU group 1 have 20 B6 － Trp53tm1 /NIFDC mice，10 male 10 female． The body weight，absolute organ weight，relative organ weight，hematological and blood biochemical parameters were measured and carried out statistical analysis．Ｒesult The decrease of the body weight of MNU group 1 and MNU group 2 compared with negative and vehicle control groups was statistically significant ( P ＜ 0. 05) ． There was statistically significant difference ( P ＜ 0. 05) of the absolute weight of heart，liver，spleen，lung，thymus and mandibular salivary gland of MNU group 1 and 2 compared with those of the negative and vehicle control groups． Furthermore，there was also statistically significant difference ( P ＜ 0. 05) of the relative weight of heart，liver，spleen，lung，kidney，brain，thymus and mandibular salivary gland of MNU group 1 and 2 compared with those of the negative and vehicle control groups． When the hematological parameters of MNU group 1 and 2 were compared with those of the 2 control groups，there were statistically significant difference ( P ＜ 0. 05) in 15 parameters，including NEU，LYM%，LUC%，ＲBC，HGB， HCT，MCV，MCHC，ＲDW，HDW，CHCM，CHDW，PDW，MPV and PLT． When the blood biochemical parameters of MNU group 1 and 2 were compared with those of the 2 control groups，there were statistically significant difference ( P ＜ 0. 05) in 7 parameters，including TP，ALB，CＲEA，UＲEA，TCHO，TG and CA． AST was only statistically significant higher in MNU group 2 compared with that of the 2 control groups ( P ＜ 0. 05) ．There was no statistically significant difference between MNU group 1 and MNU group 2． Conclusion The body weight，organ weight，hematological and blood biochemical parameters of B6 － Trp53tm1 /NIFDC mice and wild type mice treated with MNU or citrate buffer solution were measured and analyzed in the paper． The model might be used as a short-term in vivo alternative carcinogenicity study in the field of preclinical safety evaluation of drugs in the future．

Key words: <p>p53 + / － gene knockout mice, organ weight, hematological parameters, blood biochemical parameters</p>