关键词: 高脂饮食, 大鼠, 血脂, 高脂血症, 性别差异

Abstract: Abstract: Objective Effects and gender difference of high-fat diet on rat body weight and serum lipid indexes are discussed in this research． Moreover，some experimental date were provided in order to improve the specificity and reproducibility of hyperlipemia models． Method SD rats were randomly divided into 4 groups considering sexuality and diets ( normal diet or high-fat diet) ． All rats were fed for 12 weeks with given diets． Effects of highfat diets on serum total cholesterol ( TC) ，triglyceride ( TG) ，free fatty acid ( FFA) ，aspartate aminotransferase ( AST) and alanine aninotransferase ( ALT) were investigated． Ｒesult High-fat diet increased the body weight and food intake for male rats，however，body weight and food intake were decreased after high-fat diet feeding in female nate． High-fat diet expanded the gender difference on body weight． The serum contents of TC，TG，FFA， HDL-C，LDL-C in female rats were significantly increased in high-fat diet group． The serum contents of TC，FFA， HDL-C，LDL-C in male rats were also obviously increased，however，the serum TG content remain stable． Serum lipid index increased more apparently in female rats than male rats． Serum contents of AST，ALT for both sex were increased after high-fat diet feeding for 12 weeks，however，these increasing was considered as safety and controllable． Conclusion High-fat diet feeding for 12 weeks can lead to abnormal of lipid metabolism in both male and female rats with significant gender differences． Typical hypercholesterolemia ( Ⅱa type) with high body weight are observed in male rats，however，mixed hyperlipidemia ( Ⅱb type) with low body weight are founded in female rats． Gender difference should be concerned and considered in animal modeling of hyperlipidemia and therapy treatment．

Key words: high-fat diet, rats, lipids, hyperlipidemias, gender difference