关键词: 兔膝骨关节炎, 改良Hulth 法, 制备, 鉴定

Abstract: Abstract: Objective To study the preparation and identification of the model of rabbit knee osteoarthritis with the improved operational method of Hulth． Method According to the improved operational method of Hulth，rabbits were cut off knee medial collateral ligament，anterior cruciate ligament，and medial meniscus． After 8 weeks， cartilage and synovium were observed by X ray photograph，naked eye and HE staining． The expression of IL － 1、 TNF － α in serum and joint fluids were detected by Enzyme-linked Immunosorbent Assay ( ELRSA) ． Result X ray showed that obvious arthrostenosis and spur were developed． Cartilage defect and synovial inflammation were observed by naked eye． HE staining showed that cartilage defected，chondrocyte reduced and apoptosis． Compared with normal group，There were higher expression of IL － 1、TNF － αin serum and joint fluids in model group( P ＜ 0. 05) ． Conclusion After 8 weeks，the model of rabbit knee osteoarthritis with the improved operational method of Hulth was successfully made． And the model can accurately reflect the characteristic of osteoarthritis through the image analysis and biochemistry detection． It will be the theoretical basis and research groundwork for clinical treatment．

Key words: rabbit knee osteoarthritis, the improved operational method of Hulth, preparation, dentification