关键词: 心肌肽素, 心肌缺血再灌注损伤, 小型猪

Abstract: Objective To observe whether Cardiomyopeptidin can protect myocardium in a mini-pig model of acute myocardial ischemia-reperfusion injury. Methods Twelve mini-pigs were used. A balloon was inflated in the left artery descending to block the blood and result in myocardial ischemia for 90 minutes. Then the balloon was deflated to induce reperfusion injury. Sixty minutes after the balloon inflation, Cardiomyopeptidin was administered by intravenous drop infusion in test group (n=6) while 0.9% sodium chloride was used in control group (n=6). Before ischemia and at the end of and 24 hrs after administration, lactate dehydrogenase (LDH), creatine kinase (CK), and creatine kinase isoenzyme (CK-MB) in serum were detected, echocardiography was performed. Twenty-four hrs after administration, animals were sacrificed. Necrotic area was measured by triphenyhetrazolium chloride (TTC) staining and histopathology was described and scored. Apoptosis was detected by TUNEL. Results Five mini-pig of each group survived. There was no statistical difference between the two groups (P>0.05) in LDH, CK and CK-MB. The left ventricular end diastolic volume (LVEDV) of test group was increased and higher than the control group at 24 hrs after administration (P<0.05). There was no statistical difference (P>0.05) between the two groups in the rate of infarction area to all myocardium area by TTC staining and the score of myocardial injury. There were fewer apoptotic myocardial cells in test group than in control group (P<0.05). Conclusion In a mini-swine model of acute myocardial ischemia-reperfusion injury, Cardiomyopeptidin may protect myocardium by decreasing myocardial cells went apoptosis. However, the infarction area doesn’t be reduced and the function of heart doesn’t be proved only by Cardiomyopeptidin in a short-term when the myocardial injury is severe.

Key words: Cardiomyopeptidin, myocardial ischemia-reperfusion injury, mini-pig