关键词: 乙型脑炎病毒, 免疫荧光试验

Abstract: Objective Establishing the method of detecting the Japanese encephalitis virus(JEV) in swine and the biological materials from the swine with Immunofluorescence assay(IFA).Methods Selected JEV sensitive cell,titrate the virus’TCID50 and prepare the antigen slides according to GB IFA.titrate the best working density of immune fluorescence and carry out the experiments of specificity,sensitivity and stability.Result Selected BHK21 cell as JEV sensitive cell,the virus’ TCID50 is 10-8.9.mL-1;The best working density of immune fluorescence is 1∶ 200;There is no cross-reactivity with Classical swine fever virus(CSFV) and Porcine parvovirus(PPV),the experiments of sensitivity and stability show that the detection sensitivity is 1∶ 2 560 in different time;The lowest detected virus titer is 10-6.5.mL-1.Conclusion The IFA method is good in sensitivity,specificity and stability,as a result of which IFA can be used in detecting the swine and the biological materials from the swine.

Key words: Japanese encephalitis virus, Immunofluorescence assay