关键词: 小鼠, 睾丸, HE染色, 组织学观察

Abstract: To observe the development of testis in mice,the testis of total 17 groups of Kunming normal mice at their different developmental stages among 1 ～ 57 days after birth was examed by using the paraffin tissue section and HE staining. The result showed that only sertoli cells and primitive germ cells exhibited in seminiferous tubules of the early postnatal mice testis; B-type spermatogonia were observed at the 8th day after birth; Primary spermatocytes were observed at the 15th day; Secondary spermatocyte and little round spermatids appeared at the 23th day and then the quantity of round spermatids were gradually increased. At the 30th day,long-shaped sperm cells exhibited. At the 36th day,a large sum of sperms were observed in seminiferous tubules and lasted to whole subsequent stages. It means that via a process of continuously development and differentiation the primitive germ cells matured in about 36 days after birth. This study revealed more details on the development of mice testis and sperms.

Key words: Mice, Testis, HE staining, Histological observation