关键词: 胎牛成纤维细胞, 分离培养, 性别鉴定, 染色体分析

Abstract: The bovine fetal otic fibroblast cells were isolated using tissue bulk attachment. The gender of fetus was identified using PCR. The fibroblast cells from female fetus were chosen for further morphologic observation,growth curve and chromosome analyzing. The results indicated that the cells had normal morphology,multiplication characteristics and chromosome number. The establishment of culture method and related biological characterization analysis of bovine fetal fibroblast provided not only a ready supply of somatic cells for the intraspecies and interspecies cloning of macaque but also fundamental conditions for somatic transgene and other related fields.

Key words: Bovine fetal fibroblast, Isolation and culture, Sex identification, Chromosome analysis