关键词: 实验动物, 手术室, 物理灭菌(臭氧), 化学灭菌

Abstract: Objective To evaluate the efficacy,the physical disinfection method of ozone and UV was studied in the animal surgery laboratory,in which the main direction is organ transplantation of animal liver,heart and kidney.Methods In an animal clean room,where there is a day-night cycle of operation and rest,the efficacy of disinfection and sterilization was evaluated.Using culture utensils and surface microbe inspections,we compared the physical disinfection methods of ozone and UV to the chemical method of 2% Glutaral.Both sterilization processes were conducted during the night.Results Both methods achieved a normal level of sterilization after three times.However,the ozone is easy to isolate,safe to use and does not require washing.Conclusion Both Ozone and UV are physical factors.They are a good alternative to the chemical sterilization methods,as there are no waste products Also ozone is able to thoroughly permeate all parts of the equipment,including the hard to reach interior.As the needs of organ transplantation research increase,large numbers of equipment and materials need to be kept in the lab for long periods of time.Therefore it is important to establish an effective disinfection method.Our results suggest that at the correct dosage of ozone and UV,we can achieve good sterilization results in the animal surgery laboratory.

Key words: Experimental animal, Surgery laboratory, Physical sterilization(ozone), Chemical sterilization