Beagle犬免疫血清Ⅰ型腺病毒抗体检测

实验动物科学

Beagle犬免疫血清Ⅰ型腺病毒抗体检测

康纪平;李秋波;冯晓科;杨杰;

四川养麝研究所;

收稿日期:2010-02-28 修回日期:2010-02-28 出版日期:2010-02-28 发布日期:2010-02-28

Detection of Serum Antibody in Beagle Dogs Vaccinated with Canine Adenovirus Type 1

KANG Ji-ping, LI Qiu-bo, FENG Xiao-ke, YANG Jie

(Sichuan Institute of Musk Deer Breeding, Dujiangyan 611830, China

Received:2010-02-28 Revised:2010-02-28 Online:2010-02-28 Published:2010-02-28

摘要/Abstract

摘要： 目的为Beagle犬犬传染性肝炎(ICH)的预防制定合适的免疫程序提供依据。方法采用间接血凝抑制实验对24头Beagle犬(雌雄各半,20日龄～14周龄)注苗前后120份Beagle犬血清I型犬腺病毒血凝抑制抗体(HI)进行检测。结果注苗前20日龄仔犬血清母源性HI抗体效价为1:8～1∶32(平均1∶20),35日龄仔犬血清母源HI效价下降为1∶2～1∶8(平均1∶5);首免后2w(56日龄)血清HI效价上升为1∶8～1∶32(平均1∶14),二免后3w(77日龄)血清HI抗体效价上升为1∶32～1∶128(平均1∶45),三免后3w(98日龄)血清HI抗体效价继续上升为1∶64～1∶256(平均1∶79)。结论预防Beagle犬犬传染性肝炎(ICH)建议使用FiveStar犬五联弱毒疫苗,首免为35～40日龄,每间隔3w进行二免和三免加强。

关键词: Beagle犬, 犬传染性肝炎, 血凝抑制试验, 血凝抑制抗体

Abstract: Objective To establish a proper procedure for vaccination regimen against infectious canine hepatitis (ICH) in Beagle dogs.Methods Canine hepatitis hemagglutination inhibition (HI) antibody of 120 serum samples collected from 24 Beagle dogs (20-day to 14-week old) was examined by the indirect hemagglutination inhibition test.Results Maternal HI antibody titer was 1∶8-1∶32 (average 1∶20) and 1∶2-1∶8 (average 1∶5) in 20-day old and 35-day old puppy respectively. HI antibody titer was 1∶8-1∶32 (average 1∶14) 3 weeks after the first immunization, and 1∶32-1∶128 (average 1∶45) 3 weeks after the second immunization and 1∶64-1∶256 (average 1∶79) 3 weeks after the third immunization, respectively.Conclusion The Best Combinational Canine Attenuated-live Virus Vaccines are preferred to prevent canine hepatitis, and the first immunization should be performed in 35-40-day old. The second and the third immunization should be performed at an interval of 3 weeks after the first and the second immunization respectively.

Key words: Beagle dogs, Canine hepatitis, Hemagglutination inhibition test, Hemagglutination inhibition antibody

康纪平;李秋波;冯晓科;杨杰;. Beagle犬免疫血清Ⅰ型腺病毒抗体检测[J]. 实验动物科学.

KANG Ji-ping, LI Qiu-bo, FENG Xiao-ke, YANG Jie. Detection of Serum Antibody in Beagle Dogs Vaccinated with Canine Adenovirus Type 1[J]. laboratory animal science.

[1]	齐卫红，程金伟，王金照，卢难看，王书元，修中飞，王超，霍艳，李芊芊. 降糖药安全评价中至Beagle犬低血糖的兽医救治[J]. 实验动物科学, 2018, 35(01): 64-.
[2]	毕甜甜, 张晋, 文润来, 韩杰, 富强, 王小珂, 卢胜明. 实验Beagle犬贾第鞭毛虫感染情况调查[J]. 实验动物科学, 2011, 28(02): 64-.
[3]	康纪平, 李秋波, 冯晓科, 杨杰. Beagle犬部分生理指标测定[J]. 实验动物科学, 2011, 28(01): 66-67.
[4]	陈伟平,刘运忠,罗甜义. 高质量Beagle犬研究资源的科学管理[J]. 实验动物科学, 2003, 20(S1): 82-83.
[5]	巩薇,卫礼,付瑞,贺争鸣,邢瑞昌. 兔出血症病毒血凝试验及血凝抑制试验影响因素的探讨[J]. 实验动物科学, 2003, 20(03): 7-10.
[6]	黄玲,李常青,邝枣园. Beagle犬体重与各脏器重量之间关系的回归分析[J]. 实验动物科学, 2000, 17(02): 35-37.
[7]	姜北宇,郑世兰,刘月焕,白丽萍,景小冬,姚颖. 鸡传染性支气管炎HA抗原的制备及HI试验方法的建立[J]. 实验动物科学, 1998, 15(03): 0-0.
[8]	傅江南，王晓光，邱添武, 林振达，区永澄. 不同月龄Beagle犬的心电图研究[J]. 实验动物科学, 1994, 11(01): 0-0.