关键词: 昆明小鼠, 输卵管移植, 2-细胞胚胎, 输卵管壁切口移植

Abstract: Objective To compare the effects of the different oviduct embryo transfer methods,the periods and numbers of embryo transfer.Methods The different numbers of 1-cell embryo,2-cell embryo and,8-cell embryo were respectively transfefred into the oviduct of pseudopregnancy KM mouse,and the embryos were transfer to the pavilion of the oviduct tranfer and the oviduct ampullar transfer,respectively.Result The pregnancy rate of 2-cell embryo transfer is significant higher than 1-cell and 8-cell treatment group(66.67% vs 25%,33.33%,P<0.05);Transfer of 25 2-cell embryos via oviduct tranfer showed a higher birth rate than 15 and 35 2-cell embryos(36% vs 16.67%,10.71%,P<0.05);The pregnancy rate and birth rate of oviduct ampullar incision transfer were significant higher than pavilion of the oviduct tranfer(50% vs 75%,38.9% vs 49.3%,P<0.05).Conclusion Transfer of 25 2-cell embryos via oviduct ampullar incision transfer can improve the success rate of mouse embryo transfer.

Key words: KM mouse, Oviducts embryo transfer, 2-cell embryo, Oviduct ampullar incision transfer