关键词: 长爪沙鼠, DNA提取

Abstract: Objective To extract genomic DNA from different tissues of Mongolian gerbil and to compare the abundance of genes.Methods Using the phenol-chloroform method,genomic DNA was extracted from gerbil kidney,liver and muscle digested at different temperature and concentration of Proteinase K. The values of OD_(260)/OD_(280),DNA and protein were detected.Results The digesting temperature and concentration of Proteinase K had different effects on extraction of genomic DNA from kidney,liver and muscle.Digesting at 37℃and 50℃ made no difference on concentration of DNA extracted from kidney and liver,but proteins in low enzyme group were significantly lower than those in high enzyme group.The concentrations of DNA and protein of liver samples in low enzyme group were significantly lower than those in high enzyme group.Temperature had significant effect on DNA and protein concentrations of muscle samples.The abundance of genes in liver was higher than that in kidney and muscle.Conclusion Both digesting temperature and Proteinase K concentration have effects on concentrations of genomic DNA and protein extracted from different tissue samples.The abundance of genes is variant in different tissues.

Key words: Mongolian gerbil, DNA isolation, Different tissues