关键词: 基因工程小鼠, 血液常规, 血液生物化学

Abstract: Objective To determine normal range of the blood routine values and blood biochemical parameters of the genetically engineered mice(B6;129-Gt(ROSA) 26Sor).Methods Blood routine examination was performed by the automatic hematology analyzer and blood biochemical parameters were determined by the automatic biochemical analyzer in male ROSA mice aged from 5 to 6 weeks and female ROSA mice aged from 6 to 7 weeks.Results The differences in granulocyte count,percentage of granulocyte,and mean corpuscular hemoglobin were significant(P<0.05) and the differences in redcell count,hematocrit,haemoglobin and platelet distribution width were very significant(P<0.01) between female and male ROSA mice.The difference in blood creatinine level was significant(P<0.05) and the differences in albumin and total cholesterol levels were very significant(P<0.01) between female and male ROSA mice.Conclusion There are definite differences in physiological and biochemical parameters between female and male ROSA mice.

Key words: Genetically engineered ROSA mice, Blood routine, Blood biochemical parameters