关键词: 胚胎生殖细胞, 原始生殖细胞, 五指山小型猪

Abstract: Objective The experiment make a pilot study about the technique of isolating pluripotent stem cells from primordial germ cells(PGCs).Methods Fetus was derived from pregnant WuZhiShan inbreeding pigs in 26～28 d.We got fetal germ ridge,then plated on STO cells.The culture medium is composed of DMEM,2 mmol/mL L-glutamine,0.1 mmol/mL non-essential amino acid,0.1 mmol/mL β-mercaptoethanol,100 IU/mL penicillin,100 ug/mL streptomycin and 15% FCS.The embryonic germ(EG)cells was cultured and passaged.Results and Conclusions The EG cells was passaged from F_0 to F_4 or F_5.The morphology of EG cells colonies are multiplicity.By means of Alkaline phosphatase histochemical staining,Expression of stage-specific embryonic antigen-1(SSEA-1)and expression of transcription factor Oct-4 were positive.We identified that these cells possess the characteristics of embryonic stem cells.

Key words: Embryonic germ(EG)cells, Primordial germ cells(PGCs), WuZhiShan inbreeding pigs