实验动物科学

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两种抗应激剂对恒河猴抗热应激作用的试验研究

赵远;和占龙;吴海兴;李云龙;王俊斌;陈丽雄;鲁帅尧;   

  1. 中国医学科学院北京协和医学院医学生物学研究所,中国医学科学院北京协和医学院医学生物学研究所,昆明市动物疾病预防控制中心,昆明市动物疾病预防控制中心,中国医学科学院北京协和医学院医学生物学研究所,中国医学科学院北京协和医学院医学生物学研究所,中国医学科学院北京协和医学院医学生物学研究所 昆明650118,昆明650118,昆明650118,昆明650118,昆明650118,昆明650118,昆明650118
  • 收稿日期:2008-06-28 修回日期:2008-06-28 出版日期:2008-06-28 发布日期:2008-06-28

Study of The Effects of Two Anti-stressors on Rhesus Monkeys in Heat-stressed

ZHAO Yuan~1,HE Zhan-long~1,WU Hai-xing~2,LI Yun-long~2 WANG Jun-bin~1,CHEN Li-xiong~1,LU Shuai-yao~1   

  1. (1.Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming 650118,China)(2.Kunming Centres of Animal for Diseases Control and Prevention,Kunming 650118,China
  • Received:2008-06-28 Revised:2008-06-28 Online:2008-06-28 Published:2008-06-28

摘要: 目的研究两种抗应激剂对恒河猴热应激的影响。方法将6只恒河猴随机分为A,B两组采血做9项血液学及9项血液生化指标测定,A组日粮中每只添加VC300 mg/d和VE50 mg/d;B组日粮中添加0.2%苜草素,饲喂两个月后分别将A、B两组恒河猴放入(32±1)℃,湿度70%猴房内,1 h后采血,测定相应血液学及血液生化指标。结果两组动物血液学各项指标试验前后差异不显著(P>0.05);而血液生化值呈现:A组试验后AST、ALP、LDH分别为(101±23.64)U/L,(623.67±233.12)U/L,(1384.33±807.66)U/L,试验前后差异不显著(P>0.05);B组试验后TP、ALB分别为(83.83±16.32)g/L,(38.53±0.57)g/L;试验前后差异不显著。结论VC和VE在恒河猴抗热应激过程中主要作用于维持血液中AST、ALP、LDH的活性稳定,而苜草素主要作用于维持血液中TP、ALB的稳定,两种添加剂能有效提高恒河猴抗热应激的能力。

关键词: 恒河猴, 热应激, 抗应激剂, 血液学, 血液生化

Abstract: Objective Study of the effects of Two Anti-stressors on Rhesus Monkeys in heat-stressed .Methods Selected six health laboratory rhesus monkeys,and divided into group A,B,Nine hematological and nine blood biochemical were measured,A was added Vitamin C 300 mg/d and Vitamin E 50mg/d each in diets,B group was added mucaosu 0.2%,Two months later,Group A and B was bred under the condition of(32±1)℃,70% humidity for one hour,After that measured hematological and biochemical.Results Test cross-reference hematological was not significantly(P>0.05),After test,AST、ALP、LDH of group A were(101±23.64)U/L,(623.67±233.12)U/L,(1384.33±807.66)U/L and was not significant than before test(P>0.05);After test,TP、ALB of group B were(83.83±16.32)g/L,(38.53±0.57)g/L and was not significant than before test(P>0.05).Conclusion Effects of Vitamin C and Vitamin E were stood balance of AST、ALP、LDH in blood on Rhesus Monkeys in heat-stressed,Mucaosu were stood balance of TP、ALB in blood,finding two anti-stressors had effects on development anti-hotstress on rhesus monkeys.

Key words: Rhesus monkeys(Macaca mulatta), Hotstress, Anti-stressor hematological, Serum biochemical, Mucaosu