关键词: 乙肝疫苗, H-2

Abstract: Objective To use a standard stock for potency testing of HB vaccine in China.Metheods Two inbred strains(DBA/1 and BALB/c) and two NIH closes-colonies of mice were typed in the H-2 region by used microcytotoxicity method and PCR.Groups of mice of the tested strains were immunized with the same hepatitis B vaccine,the titre of anti-HBsAg antibody was analysed by microplate,the ED_(50) was then estimated bu Karder method for each strain.Results Significant differences were found between potency estimates derived from assays using different strains of mice.Conclusion It is likely that the variation of immune response to HB vaccine in mice is correlative with the H-2 haplotype.In some special case,the heterozygosity in H-2 region found in MIH stock could influence the accuracy in such testing even a reference preparation of HB vaccine was used.Base on our experiment,to select a appropriate NIH stocks with the H-2q haplotype for potency testing of HB vaccine in China.

Key words: HB vaccine, H-2, gene