犬细小病毒新抗原变异株VP2基因的克隆与序列分析

实验动物科学

犬细小病毒新抗原变异株VP2基因的克隆与序列分析

刘大伟;杨敬;范薇;隋丽华;刘宏伟;邱业峰;王鹏;赵爽;孙岩松;

军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院实验动物中心,军事医学科学院疾病预防控制所北京100071,北京100071,北京100071,北京100071,北京100071,北京100071,北京100071,北京100071,北京100071

收稿日期:2008-02-28 修回日期:2008-02-28 出版日期:2008-02-28 发布日期:2008-02-28

Clone and Sequence Analysis of New Antibody-Variant of Canine Parvovirus

LIU Da-wei~1,YANG Jing~1,FAN Wei~1,SUI Li-hua~1,LIU Hong-wei~1,QIU Ye-feng~1,WANG Peng~1ZHAO Shuang~1,SUN Yan-song~2

(1.Center of Laboratory Animal of Academy of Military Medical Science,Beijing 100071,China)(2.Institute of Disease Control and Prevention of Academy of Military Medical Science,Beijing 100071,China

Received:2008-02-28 Revised:2008-02-28 Online:2008-02-28 Published:2008-02-28

摘要/Abstract

摘要： 目的鉴定犬细小病毒(Canine parvovirus,CPV)新抗原变异株的病毒型,为研究CPV变异及制备CPV特异性诊断和治疗试剂奠定基础。方法从病毒细胞培养物中提取基因组DNA,设计合成针对VP2基因的1对特异引物,PCR扩增出VP2基因片段,克隆后测序,应用DNAstar进行序列分析。结果得到CPV-VP-2基因序列15份,其中CPV-2a 10份,CPV-2b 5份;FPV-VP-2基因1份。结论各序列与已发表的标准序列比较,同源性在98%以上,未形成明显分支。

关键词: 犬细小病毒, VP2基因

Abstract: Objective To identify the antigenic and evolution to study the mutation of CPV and lay the foundation for the identification and treatment of this disease.Methods A pair of primers were designed and synthesized based on VP2 gene of Canine parvovirus(CPV).The genomic DNA samples of CPV strain isolated from culture solution ware extracted and used as templates for PCR to clone the VP2 gene.The sequences of the DNA fragment were aligned and analyzed by DNAstar.Results 15 were CPV-VP-2,including 10 of CPV-2a and 5 of CPV-2b;1 was FPV-VP-2.Conclusions Compared with standard sequence,homogeneities were above 98%,they had not formed obvious branch.

Key words: Canine parvovirus(CPV), VP2 gene, Sequence analysis

刘大伟;杨敬;范薇;隋丽华;刘宏伟;邱业峰;王鹏;赵爽;孙岩松;. 犬细小病毒新抗原变异株VP2基因的克隆与序列分析[J]. 实验动物科学.

LIU Da-wei~1,YANG Jing~1,FAN Wei~1,SUI Li-hua~1,LIU Hong-wei~1,QIU Ye-feng~1,WANG Peng~1ZHAO Shuang~1,SUN Yan-song~2. Clone and Sequence Analysis of New Antibody-Variant of Canine Parvovirus[J]. laboratory animal science.

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