关键词: 贵州小型猪, 复合因素, 肝纤维化, 模型

Abstract: Objective To establish the hepatic fibrotic model of Guizhou minipig by multiplex factor.Methods Sixteen Guizhou minipig(8 females and 8 males) were divided into two groups including one control group and one experiment group at random.Control group was injected into peritoneum with olive oil and 0.9% NS,0.5 mL/kg twice a week,fibrosis model of experiment group was duplicated by peritoneal injection of 40% carbon tetrachloride and rabbit serum 0.5 mL/kg twice a week,for nine weeks.They were sacrificed at the end of the ninth weeks,then serum marker levels of liver function of ALT,AST,TP,ALB,A/G were determined.The liver were taken out and paraffin sections were stained by hematoxylin-eosin,Masson,Van Gieson,and were examined under light microscope(LM).The liver from experimental group were observed under electron microscopy(EM).Results Compared with the control group,serum ALT,AST activity of experiment group elevated significantly(P<0.01),ALB,A/G content decreased markedly(P<0.01);the pathological changes of liver of experiment group were observed under LM and EM.Conclusion\ Hepatic fibrosis model of Guizhou mini-pig was duplicated by peritoneal injection of 40% carbon tetrachloride and rabbit serum.

Key words: Guizhou mini-pig, Multiplex factor, Hepatic fibrosis, Model