关键词: 臭氧, 实验动物屏障环境, 消毒

Abstract: Objective The correct use of ozone research on experimental animals barrier environment for disinfection methods and concentration,to ensure that both disinfection effectiveness and environmental barriers that the experimental animals and staff security.Methods A check experiment on sterilization effects is conducted between two barriers in which the concentrations of ammonia gas are 0.32 mg/m~3and 0.32 mg/m~3.Clean ICR mice are selected for normal reproduction.The next step is to observe what influences the ozone sterilizations with different concentrations have on the birth rate and pulmonary functions of the mice as well as on the concentration of ammonia gas in the air.Results It is found that ozone sterilization does take place both when its concentration is 0.21 mg/m~3 and 0.32 mg/m~3.When the concentration of ozone is 0.32 mg/m~3,the rate of dead fetus and the case number of lung collapse of laboratory animals is obviously different from those of the control group and the group in which the concentration of ozone is 0.21 mg/m~3,and the concentration of ozone dropped 85%.When the concentration of ozone is 0.21 mg/m~3,the concentration of ozone dropped 75%,and there is no distinctive difference of the rate of dead fetus and the case number of lung collapse of animals between this group and the control group.Conclusion When the barrier environment of laboratory animals is running dynamically,the most appropriate dose of ozone disinfection is 0.21 mg/m~3.

Key words: Ozone, The barrier environment of laboratory animals, Disinfection