实验动物科学
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李鸿;陈旺;
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LI Hong,CHEN Wang
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摘要： Scid小鼠缺乏T和B淋巴细胞免疫功能,实验性糖尿病又进一步增加其感染机会,饲养难度更大。应用层流柜划分饲养及工作区,并设立“污染手制度”,在清洁环境内局部控制为SPF环境;日常饲养中适量添加维生素,成功饲养实验性糖尿病(Sevore Combined Immunodeficience Scid)小鼠四批,最长9周,顺利完成实验。实践证明,通过严密的环境控制,严格的操作程序,在清洁级环境中应用层流柜局部控制为SPF环境,短期饲养实验性糖尿病Scid小鼠是可行的。
关键词: Scid小鼠, 实验性糖尿病, 层流柜, 动物实验
Abstract: It was very difficulty breeding diabetes Scid mice for shortage of T and B lymphocyte and the animal was easy to get infection.This experiment had bred diabetes Scid mice successfully in the SPF region in streamline cabinet by measures such as:dividing breeding region and working region in streamline cabinet,dividing contaminate and clear hands,adding vitamins.It was practicable that breeding diabetes Scid mice in the SPF region in streamline cabinet short-term by accurate enviroment control and strict operating rules.
Key words: Scid mice, Scid, Streamline cabinet
李鸿;陈旺;. 应用层流柜饲养实验性糖尿病Scid小鼠体会[J]. 实验动物科学.
LI Hong,CHEN Wang. Breeding Diabetes Scid Mice with Streamline Cabinet[J]. laboratory animal science.
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