关键词: 先天性尿道下裂, 昆明小鼠, 氟丁酰胺

Abstract: Objective Through artificially interfering the pregnant mice's androgen receptors to make their male whelps suffer congenital hypospadias, and to optimize the conditions for medication time and dosage. Methods\ Female mice (Kunming species), from the 15th day of their pregnancy, received garage of fiutamide for 5 days in a dosage of 66.7mg, 133.3mg, 200.0mg,266.7mg, or 333.3mg/kg; after withdrawal, the pregnant mice ran a spontaneous labour; from on the 21st day after birth the male whelps were observed on the morphological change of their external genital processes. Results\ Group with a dosage of 133.3mg/kg or under it showed no marked abnormality in their external genital processes; group with 200.0mg/kg showed an obvious cleft occurred in the top of genital processes, with a deformity rate up to 86.6%; group with 266.7mg/kg showed their external genitals were very much similar to the female mice's, with pubes around scrotum, testes unprotruded and cryptorehid; deformity rate up to 83.3%; group with 333.3mg/kg showed a complete crack backwards in the external genitals, and even a vagina_like orifice formed in the anal side. Conclusion\ Using female mice (Kunming species), from the 15th day of their pregnancy, given a flutamide gavage in a dosage of 266.7mg/kg~333.3mg/kg for consecutive 5days may make 80% or over of congenital hypospadias models among the male mouse whelps in the experiment.

Key words: Congenital hypospadias, Mice, Kunming, Flutamide