关键词: 传染性支气管炎病毒

Abstract: Purpose To establish a convenient,specific and rapid detective technique for routine measuring antibodies to infectious bronchitis virus.Method IBV was propagated in the allantoic cavity of 9-day-old specific pathogen free(SPF),as well as in the material of chicken embryo fibroblasts(CEF)by serial passage.Followed purification,the protein quantity was measured,which was used as coated antigen.Conjugation was labeled rabbit anti-chichen IgG by using periodate method,TMB and OPD as substrate,to set up enzyme-linked immunosorbent assay(ELISA)for testing infectious bronchitis virus antibodies.Results The above two obtained IBV could be used as coating antigen.No crossing reaction was observed with antibodies to Newcastle disease virus(NDV),Marek's disease virus(MDV),infectious bursal disease virus(IBDV),infectious laryngotracheitis virus(ILTV),Avian influenza virus(AIV),Avian parainfluenza virus(APIV),Reticuloendotheliosis virus(REV),Infectious Coryza(IC),Mycoplasma gallisepticum(MG),Mycoplasma synoviae(MS).The coincidence of result is good by comparing with the imported reagent of IB-ELISA produced by IDEXX.Conclusion:The IB-ELISA diagnostic reagent can be used to detect IBV-antibody.

Key words: Infectious bronchitis virus, Enzyme-linked immunosorbent assay(ELISA)