关键词: 人, 小细胞肺癌, 裸鼠, 异种移植

Abstract: Objective To establish a successful xenotransplantation model of a human small cell lung cancer cell line (NCI_H446) in nude mice. Methods NCI_H446 cells were transplanted subcutaneously into nude mice.After the first generation,the xenografls were transplanted subcutaneously into nude mice with serial passages for three generations.The growth features,histology,ultramicroscope structure and karyology of the successfully transplanted tumors were observed. Results After four generations of transplantation the latent period was shorten, and the spontaneous regression was not observed.The growth curve of the tumor is like “S”.The karyotype of tumor cells belongs to human karyofype.

Key words: Human, Small cell lung cancer, Nude mice, Xenotransplantation