实验动物科学

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160只小鼠心脏采血的操作体会

吴晓晴,郝晨霞   

  1. 济南军区总医院动物实验中心,济南军区联勤部干休二所 济南250031 ,济南250002
  • 收稿日期:2004-03-25 修回日期:2004-03-25 出版日期:2004-03-25 发布日期:2004-03-25

Heart Puncture for Collecting Blood:The Analysis of 160 Mice

WU Xiao qing 1, HAO Chen xia 2   

  1. (1 General Hospital of Jinan Command PLA, Jinan 250031, China) (2 The Second Sanatorium for Retired officers of Service Section Army Area, Jin an 250002, China
  • Received:2004-03-25 Revised:2004-03-25 Online:2004-03-25 Published:2004-03-25

摘要: 目的 总结小鼠心脏采血的经验。方法 小鼠以氯胺酮麻醉后 ,采用 1mL注射器心脏穿刺取血。结果 小鼠心脏采血量达到 0 8~ 1 1mL。结论 心脏采血是最佳的小鼠采血方法。

关键词: 小鼠, 心脏穿刺, 采血

Abstract: Objective\ To summarize the experience in the heart puncture for collecting blood of 160 mi ce. MethodsMice were anesthetized with ketamine hydrochloride, then the heart punctures were performed by 1ml syringes.ResultsThe amount of blood collected was between 0 8~1 1mL in a heart of each mouse. ConclusionIt is the best method for collecting blood by the heart punctures in mice.

Key words: Mice, Heart puncture, Collecting blood