东方田鼠抗日本血吸虫病机制研究

实验动物科学

东方田鼠抗日本血吸虫病机制研究

刘金明,林矫矫,傅志强,李浩,魏梅雄,孙军,张亮,何艳燕,蒋守富,刘瑞三

中国农业科学院上海家畜寄生虫病研究所,农业部动物寄生虫学重点开放实验室,中国农业科学院上海家畜寄生虫病研究所,农业部动物寄生虫学重点开放实验室,中国农业科学院上海家畜寄生虫病研究所;农业部动物寄生虫学重点开放实验室,中国农业科学院上海家畜寄生虫病研究所;农业部动物寄生虫学重点开放实验室,上海市疾病预防控制中心,中国农业科学院上海家畜寄生虫病研究所,农业部动物寄生虫学重点开放实验室,中国农业科学院上海家畜寄生虫病研究所,农业部动物寄生虫学重点开放实验室,上海市疾病预防控制中心,上海市疾病预防控制中心,中国农业科学院上海家畜寄生虫病研究所,农业部动物寄生虫学重点开放实验室上海200232 ,上海200232 ,上海200232 ,上海200232 ,上海2000331 ,上海200232; ,上海200232 ,上海2000331 ,上海2000331 ,上海200232

收稿日期:2003-09-20 修回日期:2003-09-20 出版日期:2003-09-20 发布日期:2003-09-20

Study on the Mechanism of Microtus fortis Against Schistosoma Japonicum

LIU Jin ming 1, LIN Jiao jiao 1, FU Zhi qiang 1, LI Hao 1, WEI Mei xiong 2, SUN Jun 1, ZHANG Liang 1, HE Yan yan 2, JIANG Shou fu 2, LIU Rui san 1

(1. Shanghai Institute of Animal Parasitology, CAAS, The Key Open Laboratory for Animal Parasitology, Ministry of Agriculture, Shanghai 200232,China) (2. Shanghai Municipal Center of Disease Control and Prevention, Shanghai\ 200031,China

Received:2003-09-20 Revised:2003-09-20 Online:2003-09-20 Published:2003-09-20

摘要/Abstract

关键词: 东方田鼠, 血吸虫

Abstract: This paper was about the study on the mechanism of Microtus fortis against Schistosoma japonicum. Firstly, we confirmed that Microtus fortis came from epidemic region (Dongting Lake beaches) and non epidemic region (Qingtong Gorge in Ningxia province) were both resistant to Schistosoma japonicum infection after re infection tests for several times. It seemed that their resistant ability was inheritable rather than acquired. Secondly, it was demonstrated by in vivo check up and in vitro killing assay that there were some native antibodies of IgG3 subclass specifically to the schistosomula and adult worm of Schistosoma japonicum in Microtus fortis, which probably played an important role in resisting Schistosoma japonicum associated with complement. It was shown that macrophages and eosinophils in abdominal cavity of Microtus fortis had native ability of adhering to the schistosomula of Schistosoma japonicum. Then, the adult worm cDNA library of Schistosoma japonicum was screened with sera from Microtus fortis . Five positive clones were obtained, four of which were identified as new genes. Full length cDNA of the two new genes were isolated by RACE. DNA vaccine was constructed with one named EST mfs 3. After the Kunming mice immunized with this vaccine, the worm reduction rate and the egg reduction rate were 28.4% and 21.73% compared with that in control group respectively. This kind of DNA based EST mfs 3 vaccine was highly expressed in E.coli and induecd strong immune response in challenged group. Finally, two groups of cDNA probes prepared from liver and lung of Microtus fortis with or without Schistosoma japonicum infection were hybridized to the cDNA chip prepared from rat respectively. 156 and 332 genes revealed differential expression in infectious group compared with normal group. In conclusion, there would be many factors contribute to the mechanism of Microtus fortis against Schistosoma japonicum. We should stress the essentials and make further research on how to take advantage of them to defend us from Schistosoma japonicum infection.

Key words: Microtus fortis, Schistosoma japonicum, Mechanism

刘金明,林矫矫,傅志强,李浩,魏梅雄,孙军,张亮,何艳燕,蒋守富,刘瑞三. 东方田鼠抗日本血吸虫病机制研究[J]. 实验动物科学.

LIU Jin ming 1, LIN Jiao jiao 1, FU Zhi qiang 1, LI Hao 1, WEI Mei xiong 2, SUN Jun 1, ZHANG Liang 1, HE Yan yan 2, JIANG Shou fu 2, LIU Rui san 1. Study on the Mechanism of Microtus fortis Against Schistosoma Japonicum[J]. laboratory animal science.

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