实验动物科学

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兔出血症病毒血凝试验及血凝抑制试验影响因素的探讨

巩薇,卫礼,付瑞,贺争鸣,邢瑞昌   

  1. 中国药品生物制品检定所,中国药品生物制品检定所,中国药品生物制品检定所,中国药品生物制品检定所,中国药品生物制品检定所 北京100050 ,北京100050 ,北京100050 ,北京100050 ,北京100050
  • 收稿日期:2003-09-25 修回日期:2003-09-25 出版日期:2003-09-25 发布日期:2003-09-25

Analysis of Factors Affecting the Hemagglutination and Hemagglutination-inhibition Test for Detection of Antibody to Rabbit Hemorrhagic Disease Virus

GONG Wei, WEI Li, FU Rui, HE Zheng\|ming, XING Rui\|chang   

  1. (National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050
  • Received:2003-09-25 Revised:2003-09-25 Online:2003-09-25 Published:2003-09-25

摘要: 目的 探讨影响血凝、血凝抑制实验结果的各种可能因素。方法 比较 7种微量反应板在不同稀释系统和不同血清处理方法情况下进行血凝和血凝抑制试验的结果。结果 找出了图谱清晰 ,反应结果规律、稳定的实验条件。结论 为我国国标的进一步完善提供了实验依据。

关键词: 兔出血症病毒, 血凝试验, 血凝抑制试验

Abstract: Objective\ To explore the factors that affect the hemagglutination and hemagglutination\|inhibition test. Method\ Hemagglutination and hemagglutination\|inhibition test in different dilution and different serum treatments and 7 kind of reactive plates were compared. Result\ The test condition of clear image, regular and stable result was obtained. Conclusion\ The results provided some experimental data for fine instructions of the national standard.

Key words: Rabbit Hemorrhagic Disease Virus, Hemagglutination test, Hemagglutination\|inhibition test