重组戊型肝炎疫苗的免疫原性试验

实验动物科学

重组戊型肝炎疫苗的免疫原性试验

陈献花,楼琦,刘金明,金晓音

浙江省实验动物中心,浙江省实验动物中心,浙江省实验动物中心,浙江省实验动物中心杭州310013 ,杭州310013 ,杭州310013 ,杭州310013

收稿日期:2003-09-25 修回日期:2003-09-25 出版日期:2003-09-25 发布日期:2003-09-25

Immunologenic Charaterization of Recombinant Hepatitis E Vaccine

CHEN Xian\|hua, LOU Qi, LIU Jin\|ming,JIN Xiao\|yin

(Zhejiang Laboratory Animal Center,Hangzhou\ 310013

Received:2003-09-25 Revised:2003-09-25 Online:2003-09-25 Published:2003-09-25

摘要/Abstract

摘要： 目的　研究重组戊型肝炎疫苗的免疫原性。方法　通过对小鼠、新西兰兔的多点皮内注射及肌肉注射 ,然后测定其抗体效价 ,研究重组戊型肝炎疫苗的性能。结果　小鼠在免疫 7d后开始产生抗体 ,两周抗体阳性率为80 % ,四周抗体阳性率达 10 0 %。新西兰兔从第二周后开始产生抗体 ,第三周达到高峰 ,抗血清在 1∶6 4 0 0稀释度的阳性率达 4 0 %。结论　重组戊型肝炎疫苗免疫动物具有良好的免疫原性 ,为进一步研究开发戊肝疫苗对人体的免疫提供了基础

关键词: 戊型肝炎病毒, 重组疫苗, 免疫原性

Abstract: Objective\ To study immunologenic characteristics of recombinant hepatitis E vaccine.Methods\ Mice and rabbits were immunized with recombinant hepatitis E vaccine by multipoint intradermal injections and muscle injections,then the serum antibody titer was measured.Resaults\ The serum antibody was detected 7 days after mice immunized.The positive rate was 80% after 2 weeks and 100% after 4 weeks. In rabbits,antibody was detected after 2 weeks and reached the peak after 3 weeks,the serum antibody positive rate was 40% on 1∶6400 dilution.Conclusion\ The results indicated that the recombinant hepatitis E vaccine had good immunogenic and can be used for hepatitis E vaccine research.

Key words: Hepatitic E virus, Recombinant vaccine, Immunological character

陈献花,楼琦,刘金明,金晓音. 重组戊型肝炎疫苗的免疫原性试验[J]. 实验动物科学.

CHEN Xian\|hua, LOU Qi, LIU Jin\|ming,JIN Xiao\|yin. Immunologenic Charaterization of Recombinant Hepatitis E Vaccine[J]. laboratory animal science.

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