关键词: 肺支原体

Abstract: Preliminary quality comparison between 2 brand (Tiantan and Difco) artificial media for mycoplasma pulmonis has been conducted. No significant quality difference has been observed between them. For both media, the soft-agarose culture method has been proved to grow 100ul inoculum of 10 -7 dilution of positive sample while that of broth culture has been proved to grow 100ul inoculum of 10 -9 dilution of the same sample in terms of sensitivity test. When these 2 artificial medias are used for quantifying living mycoplasma cells in a control sample, Tiantan media gives rise to 8 5×10 -8 CFU/ml while Difco gives 1×10 -9 CFU/ml. When the 2 medias are used for storing mycoplamsa under -20℃, the activity of the organism decreases 1 Log level within 2 months, but declines sharply after 6 months. The growth and development of mycoplasma pulmonis on Difco medium are superior to Tiantan media.

Key words: Mycoplasma pulmonis, Artificial media