关键词: 肝癌H_(22), SDS聚丙烯酰胺凝胶电泳, 免疫组化

Abstract: Objective: To compare and research the protein composition of H 22 cell lines from different institutes.Methods: H 22 cell lines attained from three institutes were kept in abdominal cavity of KM mouse.The protein composition of different H 22 cell lines were compared with SDS polyacrylamide gel electrophoresis (SDS\|PAGE) and immunohistochemistry methods.Results: SDS\|PAGE electrophoresis showed that the numbers of protein bands of H 22 from different institutes were different,and there were five bands that their pigmentary intensities markedly varied,which indicated that the corresponding protein contents in different H 22 cell lines were different. The protein composition of PDGF\|A,TrkA,Gai,CDk2,NFκ Bp65 showed qualitative difference ( p <0.01).Conclusion: There were obvious differences among the different H 22 cell lines in the protein composition.

Key words: Murine Hepatoma 22, SDS\|PAGE, immunohistochemistry