关键词: 犬, 卵母细胞, 体外受精

Abstract: Received ovaries from estrous dogs and diestrous dogs. The oocytes derived from >2 mm and 1-2 mm antral follicles on estrous dogs and dietrous dogs. These oocytes were inseminated for 12h. The results were described as follows:\;①In the estrous group, the monospermy rate(31.2 %), pronuclei formation rate(19.4 %) were higher than that in the diestrous group and the polyspermy rate(12.6 %) was lower than that in the diestrous group. ②The monospermy rate of the antral follicles groups of 1-2 mm and >2 mm were higher than that of the invisible follicles group, and the pronuclei formation rate were higher than that of the invisible follicles group. ③The cleavage rate of >2 mm antral follicles was higher that of the oocytes derived from invisible follicles inestrous ovaries and diestrous ovaries. the clevage rates of oocytes derived from invisible follicles in estrous ovaries and in diestrous ovaries were no difference.

Key words: Dog, Oocyte, In vitro fertilization