关键词: 放射, β-胡萝卜素, 鼻咽癌, 裸鼠, 流式细胞计

Abstract: The effects of radiation and/or β-carotene on the 36th passage human nasopharyngeal carcinoma xenografts in nude mice were studied by tumor volume and flow cytometry. The xenografts were implanted subcutaneously into the posterior limb of the animals, β-carotene (12mg/kg, p.o., q.d.) and/or a single does of 5 Gy ~(60)Co γ-ray was given to the nude mcie, and 0.9%NaCl (4mg/kg, p.o., q.d.) was used as control group. In 4 weeks after treatment, the relative tumor volume in radiation or radiaton combined with β-carotene goup. The factorial analysis indicated that radiation was the main factor of inhibiting tumor growth (p<0.05 or 0.01), but β-carotene had no statistically effect on tumor growth (p<0.05). The flow cytometric analysis showed that the S% of tumors in the grouos of control, β-carotene, radiation and radiation combined with β-carotene was 10.0, 9.9, 14.4 and 15.9% respectively. It indicated that radiation cause a significant rise in S% (P<0.01) and G_2+ M%(P<0.05). The S% of bone marrow in above 4 groups was 10.1 ,13.7,16.8 and 15.0% respectively; and G_2+M% was 11.0, 12.7, 17.4 and 14.2% respectively. A significant rise of G_2+M% (P<0.05) in bone marrow had been obtained by radiation, but the effect was inhibited by β-carotene (P<0.05).

Key words: Radiation, β-carotene, Nasopharyngeal Carcinoma, Nude Mice, Flow Cytometry