关键词: 免疫缺陷动物, 肿瘤细胞侵袭

Abstract: In recent 10 years, we have employed immuno-deficiency animal to observe the invasiveness of human tumors from different point of view. In summary, the following works were undertaken. (1) Establishment of animal models of invasion, six in vivo iuvasion models were set up,including dorsal-lateral subcutaneous, intramuscular, intra-peritoneal, subrenal capsule, subtesticular capsule and footpadal subcutaneous imptantation. These models could be used for different purpose. All the six models could be employed for comparative study of tumor invasiveness. (2) Observation on the relationship between invasion and metastasis. The footpadal subcutaneous implantation model was used in the study. Local tumor was eradicated at different time interval. The relationship between local invasion and metastasis of tumor were inve-stigatedeither by sacrifying the animal at different time or after keeping the animal as long as they could survive. Our results indicate that metastasis can form only after local invasion reached Ⅲ-degree. So Ⅲ-degree local invasion is the critical stage. (3) Primary evaluation of quantitation of tumor invasion. (4) Observation on the relationship between the invasiveness of tumor cells and organ microenviroment. The results suggested the invasiveness of tumor cells, like metastasis of them, is also organspecific. (5) Our investigation also proved that the expression of tumor cell invasion is closely related with the ways they were implanted. In a word, our experimental results in 10 years were summed up and analysed. Problems worth further investigation was raised. These observations lay good foundation for further study of the mechanism and experimental treatment of tumor cell invasion.

Key words: Immunodeficiency animal, Tumor invasion