多瘤病毒的培养特性及血清抗体的检查

实验动物科学

多瘤病毒的培养特性及血清抗体的检查

涂新明;蒋虹;吴小闲;

中国医学科学院实验动物研究所微生物免疫室,中国医学科学院实验动物研究所微生物免疫室,中国医学科学院实验动物研究所微生物免疫室北京潘家园南里 100021,北京潘家园南里 100021,北京潘家园南里 100021

收稿日期:1992-09-25 修回日期:1992-09-25 出版日期:1992-09-25 发布日期:1992-09-25

The Cultural Characteristics and Serodiagnosis of Polyoma Virus

Tu Xinming Jiang Hong Wu Xiaoxian Institute of Laboratory Animal Science, Chinese Academy of Medical Science Beijng, 100021

Received:1992-09-25 Revised:1992-09-25 Online:1992-09-25 Published:1992-09-25

摘要/Abstract

摘要： 多瘤病毒最适宜的培养条件为接种病毒吸附1.5h,培养第4天换液1次,用无牛血清维持液培养12天,其血凝素达高峰。当培养物出现血凝素时,即有细胞内抗原的存在。应用血凝抑制试验(HI)和酶免疫法(EIA)两种方法进行对比性试验,检查实验感染多瘤病毒的10只SPF小鼠和192只普通小鼠血清多瘤病毒抗体的出现,实验结果表明HI较EIA敏感。多瘤病毒存在于我国普通饲养的实验小鼠群中。

关键词: 细胞内抗原, 多瘸病毒血清抗体, 种毒吸附

Abstract: The optimum culture conditions for polyoma virus is that the polyoma virus adheres to the mouse embryonic cells for 1.5 hours after inoculation, changing the medium on 4th day, and culturing in non-calf serum maintenanee medium for more than 8 days. There has been intercellular antigen while the hemagglutinin appeared in medium. A comparative test was carried out by hemagglutination inhibition (HI) test and immunoenzymatic assay (IEA) for antibody of polyoma virus on 10 SPE mice and 192 conventional mice The results shows that HI is more senstive than IEA and the serum positive for polyoma virus antibody in conventional mice, but negative in SPF mice.

Key words: polyoma virus antibody, mouse embryonic cells

涂新明;蒋虹;吴小闲;. 多瘤病毒的培养特性及血清抗体的检查[J]. 实验动物科学.

Tu Xinming Jiang Hong Wu Xiaoxian Institute of Laboratory Animal Science, Chinese Academy of Medical Science Beijng, 100021. The Cultural Characteristics and Serodiagnosis of Polyoma Virus[J]. laboratory animal science.