实验动物科学 ›› 2024, Vol. 41 ›› Issue (5): 23-29.DOI: 10.3969/j.issn.1006-6179.2024.05.005

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改良特异性皮炎小鼠模型的建立与评估

  

  1. ( 1. 西安市院士工作站,基础与转化医学研究所,西安医学院,西安 710021) ( 2. 西安市常见老龄疾病防治重点实验室,
    慢病防治研究与转化中心,基础与转化医学研究所,西安医学院,西安 710021)
  • 收稿日期:2023-07-28 出版日期:2024-10-28 发布日期:2024-10-28
  • 通讯作者: 栾 晶( 1988—) ,女,副教授,研究方向为自身免疫性疾病的发病机制研究,E-mail:luanjing666@ 126. com。
  • 作者简介:武 强( 1998—) ,女,硕士,研究方向为特异性皮炎发病机制研究,E-mail:wqwyyx57@ 163. com。
  • 基金资助:
    陕西省重点研发计划项目( 2021ZDLSF02-09)

Establishment and Evaluation of Mouse Model of Modified Atopic Dermatitis

  1. ( 1. Xi’ an Academician Workstation, Institute of Basic and Translational Medicine, Xi’ an Medical University, Xi’ an 710021, China)
    ( 2. Xi’ an Key Laboratory for the Prevention and Treatment of Common Aging Diseases, Chronic Disease Prevention Research
    and Transformation Center, Institute of Basic and Translational Medicine, Xi’ an Medical University, Xi’ an 710021,China)
  • Received:2023-07-28 Online:2024-10-28 Published:2024-10-28

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摘要：

目的 对现有的建模方法和评价指标进行优化以提高模型的成功率和实验结果的可靠性,同时,对 BALB / c
和 C57BL / 6J 两种不同品系的小鼠特异性皮炎模型进行了对比评价。 方法 8 ~ 10 周龄 BALB / c 和 C57BL / 6J 小鼠
各 16 只,分为对照组和模型组(每组 8 只) ,使用 2,4-二硝基氯苯( DNCB) 诱导小鼠产生特异性皮炎( AD) 样改变,
动态观察并统计小鼠皮炎发病程度,抓挠次数和体质量等,在实验观察的终点收集对照组和模型组小鼠脾指数,对
外周血免疫细胞变化和血清相关炎症因子水平等相关数据进行统计分析。 结果 实验第 3 天,两种品系模型组小
鼠均开始出现皮肤肿胀、红斑等皮炎表型,随时间进展皮炎症状进一步加重。 与同品系对照组小鼠相比,各模型组
小鼠有明显的皮炎表现,脾指数、抓挠次数、外周血免疫细胞、相关炎症因子水平均高于其对照组水平。 BALB / c 小
鼠的皮炎表型更易观察,但各指标与 C57BL / 6J 小鼠有较大差异。 结论 两种品系小鼠都可用于 AD 小鼠模型构
建。 与 C57BL / 6J 小鼠相比,BALB / c 小鼠的皮炎症状更为明显,皮炎恢复能力弱。

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Abstract:

Objective To improve the model’ s success rate and the reliability of the experimental result,
the existing modeling method and evaluation indexes were optimized, and a comparative evaluation of two
different strains of mouse-specific dermatitis models, BALB / c and C57BL / 6J, was performed. Method
At the age of 8 - 10 weeks, BALB / c and C57BL / 6J mice were divided into control and model groups
( n = 8) . 2, 4-dinitrochlorobenzene (DNCB) was used to induce specific dermatitis (AD) -like changes
in mice. The incidence degree of dermatitis, the number of scratching and body weight of mice were
dynamically observed and counted. The spleen index, peripheral blood immune cells, and serum levels of
inflammatory factors were collected from control and model mice at the end of the experiment for statistical
analysis. Result On the third day of the experiment, mice from both strains developed skin swelling,
erythema, and other dermatitis phenotypes, which worsened as the experiment progressed.
When compared to control mice of the same strain, the mice in each model group had significant dermatitis, as 
well as higher levels of the splenic index, scratching frequency, peripheral blood immune cells, and
relevant inflammatory factors. The dermatitis phenotype was more visible in BALB / c mice, but the spleen
index, scratching frequency, peripheral blood immune cells, and dermatitis score differed from C57BL /
6J mice. Conclusion The two strains of mice can be used in the development of AD mouse models.
However, the BALB / c mice demonstrated a greater incidence of dermatitis and displayed a slower rate of
recovery than the C57BL / 6J mice.

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