小鼠肺炎病毒, 口咽拭子, 消长规律

Objective To evaluate the applicability of molecular pathogen detection and serological testing method in the detection of pneumonia virus of mice ( PVM) . Method BALB / c and C57BL / 6J mice, half male and half female, aged 3 weeks, were infected with PVM via nasal droplets. Oro pharyngeal swabs and tail tip blood were collected from each experimental mouse at 1 week, 2 weeks, 3 weeks, 1 month, 2 months, and 3 months post-infection, and were tested using PCR and ELISA method. Result One week after infection with PVM, the detection rate of PVM nucleic acid in oropharyngeal

swabs of mice from both strains was 70% to 100%, which decreased and had higher CT values in the

following 2 weeks and 3 weeks, with no detection after 1 month; peripheral blood PVM antibodies were

not detected in the first week, but the detection rate was 80% to 100% in the following 2 weeks and 3

weeks, with all being detected from 1 to 3 months, and the optical density of PVM antibodies detected by

ELISA continued to increase, maintaining a high antibody level in the 2nd and 3rd months. Conclusion

PCR detection of oropharyngeal swab samples combined with serological testing technology can be applied

to the quality control of PVM in mice.

PVM, oral swab, grows and decline regularity