关键词: 比格犬, 微卫星 DNA, 群体遗传结构, 遗传信息, 遗传检测

Abstract: To analyze the genetic structure of outbred populations of small Beagle dogs from variant locations using the selected microsatellite loci, and evaluate if these markers are suitable for genetic detection of the outbred Beagle dog. Method A total of 20 microsatellite markers that has been verified were used to amplifying and STR scanning the sample from 3 companies ( Xi’ an, Beijing and Jiangsu) outbred populations of small beagle dogs by optimized PCR conditions. After genotyping, the genetic indexes were calculated and genetic population structure were analyzed. Result The number of average effective allele of Xi’ an, Beijing and Jiangsu outbred populations of small beagle dogs exhibited with 5. 45, 6. 80, 6. 10, respectively; average heterozygosity was 0. 585 2, 0. 653 8, 0. 663 1, respectively, Shannon’ s information index was 1. 177 4, 1. 384 4, 1. 349 8 and PIC was 0. 539 7, 0. 614 7, 0. 618 1, respectively. It showed that 3 examined population displayed high and abundant genetic population polymorphism and high genetic diversity. The 20 microsatellite loci we used could reflect the general genetic polymorphism character of each population, as well as its unique feature. Conclusion Three outbred populations of small beagle dogs harbor comparably high polymorphism and match the demand of outbred population animal. The set of 20 microsatellite loci we optimized could be adapted for genetically monitoring outbred Beagle dogs.

Key words: :Beagle dogs, microsatellite DNA, population genetic structure, genetic information, genetic detection