自发持续性心房颤动动物实验模型的探索研究

doi:10. 3969 / j. issn. 1006-6179. 2023. 03. 010

实验动物科学 ›› 2023, Vol. 40 ›› Issue (3): 61-70.DOI: 10. 3969 / j. issn. 1006-6179. 2023. 03. 010

自发持续性心房颤动动物实验模型的探索研究

( 1. 北京积水潭医院,胸外科北京 100037) ( 2. 中国医学科学院,北京协和医学院,国家心血管病中心,阜外医院,心血管疾病国家重点实验室,心血管植入材料临床前评价北京市重点实验室,动物实验中心,北京 100037)

收稿日期:2022-09-05 出版日期:2023-06-28 发布日期:2023-07-05
通讯作者: 李彬( 1987—) ,男,副研究员,研究方向:动物实验及相关研究. E-mail:libin@ fuwai. com
作者简介:张冬( 1986—) ,男,主治医师,研究方向:胸心外科的基础及临床相关研究. E-mail:xiaoyuanxingyu@ 126. com

Experimental Study on Animal Models of Spontaneously Persistent Atrial Fibrillation

( 1. Department of Thoracic Surgery, Beijing Jishuitan Hospital, Beijing 100037,China) ( 2. Chinese Academy of Medical Sciences, Peking Union Medical College,National Center for Cardiovascular Disease,Fuwai Hospital,State Key Laboratory of Cardiovascular Disease, Beijing Key Laboratory of Pre-Clinical Research and Evaluation for Cardiovascular Implant Materials, Animal Experimental Center,Beijing 100037,China)

Received:2022-09-05 Online:2023-06-28 Published:2023-07-05

摘要/Abstract

摘要： 目的探索建立自发持续性房颤的动物实验模型。方法选择贵州小型猪,经麻醉诱导、呼吸插管后建立二尖瓣返流及假手术组模型。二尖瓣返流模型组 4 只,对照假手术组 4 只,对比观察术后最长周期(6 个月)内自发持续性房颤的发生与发展,期间(术前、术后即刻、术后 3 个月、术后 6 个月)分别进行心电图及心脏超声检测,以便明确房颤及瓣膜返流情况。期间模型若出现自发持续性房颤,实验即刻终止,并进行三维电生理标测;若实验动物模型未出现自发持续性房颤,最长观察时间为 6 个月,并进行三维电生理标测及房颤易感性检验。结果成功建立二尖瓣返流及假手术组模型,各组最长周期(6 个月)内均未出现自发持续性房颤;二尖瓣返流组左心房明显扩大,出现明显结构重构;与对照组相比,二尖瓣返流组短时间房颤片段显著减少,起搏刺激后房颤心率 / 全部心率比例显著增加;二尖瓣返流组与对照组相比房颤易感性明显增高。结论本实验成功建立持续性房颤的动物实验模型1 例,并发现二尖瓣返流可以引起房颤易感性的明显升高。

关键词: 心房颤动, 二尖瓣返流, 动物模型

Abstract: Objective To establish an animal model of spontaneous persistent atrial fibrillation. Method Guizhou miniature pigs were selected and the mitral regurgitation and sham operation group models were established after anesthesia induction and respiratory intubation. Mitral regurgitation model group ( 4 cases) and sham operation group ( 4 cases ) were compared to observe the occurrence and development of spontaneous persistent atrial fibrillation within the longest postoperative period ( 6 months) . During the period ( before surgery, immediately after surgery, 3 months after surgery, 6 months after surgery ) , electrocardiogram and cardiac ultrasound were performed respectively, so as to clarify the situation of atrial fibrillation and valve regurgitation. If spontaneous persistent atrial fibrillation appeared in the model during the period, the experiment was immediately terminated and three-dimensional electrophysiological mapping was performed. If there was no spontaneous persistent atrial fibrillation in the experimental animal model, the longest observation time was 6 months, and three-dimensional electrophysiological mapping and susceptibility test of atrial fibrillation were performed. Result The model of mitral regurgitation and sham operation group was established successfully. No spontaneous persistent atrial fibrillation occurred in the longest period ( 6 months) of each group. In the mitral regurgitation group, the left atrium was obviously enlarged and showed obvious structural remodeling. Compared with the control group, the fraction of short atrial fibrillation in the mitral regurgitation group was significantly reduced, and the ratio of heart rate to total heart rate of atrial fibrillation after pacing stimulation was significantly increased. The mitral regurgitation group was significantly more susceptible to atrial fibrillation than the control group. Conclusion An animal model of persistent atrial fibrillation was successfully established in this study, and it was found that mitral regentation can significantly increase the susceptibility to atrial fibrillation.

Key words: atrial fibrillation, mitral regurgitation, animal model

中图分类号:

Q95-3

张冬, 张敏, 朱瑾彦, 李彬. 自发持续性心房颤动动物实验模型的探索研究[J]. 实验动物科学, 2023, 40(3): 61-70.

ZHANG Dong , ZHANG Min , ZHU Jinyan , LI Bin. Experimental Study on Animal Models of Spontaneously Persistent Atrial Fibrillation[J]. Laboratory Animal Science, 2023, 40(3): 61-70.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: http://www.sydwkx.com/CN/10. 3969 / j. issn. 1006-6179. 2023. 03. 010

http://www.sydwkx.com/CN/Y2023/V40/I3/61

http://m.chaoxing.com/mqk/read_38502727e7500f26df08404b3bda43fa02e092b64cf4811c1921b0a3ea255101fc1cf1fbb4666ae63ca29bba4b44f9c2c44a7a19fa747bd1840c10f83976136003f8c197b0be512a9ee42e536699d26b?from=screen

[1]	张冬, 朱瑾彦, 张敏, 李彬. 扩张型心肌病动物模型的建立与评估[J]. 实验动物科学, 2023, 40(4): 55-61.
[2]	谭杰仁, 王晓明, 程旭, 折志刚, 张晓晶, 李红良, 张娥娟. 犬乳腺癌基因表达特征及肿瘤预后标志物分析[J]. 实验动物科学, 2023, 40(3): 42-52.
[3]	姚智超, 朱妍, 霍丽蓉. 血管内膜增生和再狭窄手术模型的研究进展[J]. 实验动物科学, 2023, 40(3): 104-108.
[4]	张入峰, 熊雪阳, 李雪成, 牛云超, 王志文, 喻长远. 基于 ob / ob 小鼠的非酒精性脂肪肝模型构建方法比较[J]. 实验动物科学, 2023, 40(2): 10-16.
[5]	欧琅琳, 王朝思, 梅健, 朱祥瑞, 马翠. 关于医学实验动物模型构建中的疼痛管理及动物福利的思考[J]. 实验动物科学, 2023, 40(2): 82-88.
[6]	曹玲丽, 徐为公. 兔创伤性出血模型的建立方法[J]. 实验动物科学, 2023, 40(2): 89-93.
[7]	倪艳, 是文辉, 曹彦, 李佳佳, 李建瑛, 董翔, 赵红琼, 许琴. 功能性肠病大鼠模型两种造模方法的比较[J]. 实验动物科学, 2023, 40(1): 55-60.
[8]	郑心茹, 张晓清, 杨洋, 王吉魁, 韩雪. 2,4-二硝基氯苯诱导 BALB / c 小鼠特应性皮炎模型的建立[J]. 实验动物科学, 2022, 39(6): 57-62.
[9]	王晓菲, 严语, 赵展, 何沐, 王璟萱, 武军驻, 杨怀洁. 激素模拟妊娠后激素停撤大鼠产后抑郁模型的改进与评价[J]. 实验动物科学, 2022, 39(4): 13-17.
[10]	朱喜春, 张永兴, 俞发荣. 不饱和脂肪酸对肝癌移植瘤小鼠 P21、P53、P13K 及 PKB / Akt 蛋白表达的影响[J]. 实验动物科学, 2022, 39(4): 33-38.
[11]	刘薇薇, 于洋, 闻德亮. 慢性昼夜节律紊乱实验动物造模及其致病机制研究进展[J]. 实验动物科学, 2022, 39(4): 59-63.
[12]	吕海燕, 侯广玉, 朱梅. MPTP 致帕金森病动物模型的研究进展[J]. 实验动物科学, 2022, 39(4): 64-68.
[13]	任哲郯, 谢雨瑶, 刘安琪. 药物诱导斑马鱼心力衰竭模型的研究进展[J]. 实验动物科学, 2022, 39(4): 74-78.
[14]	马艳芳, 张鑫, 李慧, 程东, 孙昌华. 骨质疏松症动物模型中大鼠卵巢切除手术要点及其术后护理[J]. 实验动物科学, 2022, 39(3): 57-62.
[15]	李建香, 王君君, 黄天马, 张子健, 过伟峰. 无菌鼠模型制作方法与评价[J]. 实验动物科学, 2022, 39(3): 67-69.

自发持续性心房颤动动物实验模型的探索研究

Experimental Study on Animal Models of Spontaneously Persistent Atrial Fibrillation

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics