关键词: 巴马小型猪, 高脂高糖饮食, 肠道菌群

Abstract: Objective To explore the effect of a high-fat and high-sucrose diet on the blood lipid and gut microbiota of the Bama miniature pigs. Method A total of 10 Bama miniature pigs were selected and randomly divided into control group ( n = 3) and experimental group ( n = 7) . Animals in control group were fed with basic diet and in experimental group with high-fat and high-sucrose diet. Fasting blood lipids, including triglyceride, total cholesterol, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol, were detected before the experiment and in the 8th month. Meanwhile, 16S rRNA sequencing was performed to detect the composition of gut microbiota. Result After 8 months of feeding with high-fat and high-sucrose diet, the number of gut microbiota did not change significantly, however, the relative abundance of Firmicute increased, while the abundance of Bacteroide and Proteobacteria decreased in the experimental group (P<0. 05) . Moreover, the body weight and the level of triglyceride, cholesterol and low-density lipoprotein cholesterol of the experimental group were significantly higher than those of the control group ( P< 0. 05) . Conclusion High-fat and high-sucrose diet could induce the increasement of Firmicute, and the reduction of Bacteroide and Proteobacteria in the feces of Bama miniature pigs, suggesting that the imbalance of these gut microbiota may be involved in regulating the occurrence of hyperlipidemia in the host.

Key words: Bama miniature pigs, high-fat and high-sucrose diet, gut microbiota