关键词: 胸腺, 兔, 取材方法 

Abstract: Objective To observe the developmental involution process of the rabbit thymus and the precautions when harvesting it. Method Eight large ear white rabbits were obtained and randomly divided into a blank group and an immunosuppressive model group with four rabbits in each group. The immunosuppression model was prepared with cyclophosphamide in the model group, which was injected intraperitoneally at 60 mg / kg for 7 consecutive days. Twenty days after the end of modeling, thymus tissues were harvested for HE staining. Result The general morphology showed that the thymus was mostly bilobed on the left and right, with a hard texture and more tough than adipose tissue. Among them, the thymus of the blank group was mostly light pink, and the thymus of the cyclophosphamide model group was mostly white. HE result showed that the thymus was structurally intact, the corticomedullary boundary was clear, and the cortex was densely lymphocytic; in immunosuppression model group, the thymus lost its normal structure, the corticomedullary boundary was unclear, the cortex was lymphopenic, and the surrounding adipose tissue was more. Conclusion The immunosuppression model caused by cyclophosphamide would produce irreversible destruction to the morphological structure of thymus tissue in the organism.

Key words: thymus, rabbit, material method