对改造的阿霉素肾病模型的评价

实验动物科学

对改造的阿霉素肾病模型的评价

鲁斌,李新民,马融

天津中医学院第一附属医院分子生物学实验室!天津!300193,天津中医学院第一附属医院分子生物学实验室!天津!300193,天津中医学院第一附属医院分子生物学实验室!天津!300193

收稿日期:1999-09-25 修回日期:1999-09-25 出版日期:1999-09-25 发布日期:1999-09-25

Evaluation of the Modified Adriamycin Induced Nephrotic Rats

Lu Bin, Li Xinmin, Ma Rong First Teaching Hospital of Tianjin Traditional Chinese Medical College 〔

Received:1999-09-25 Revised:1999-09-25 Online:1999-09-25 Published:1999-09-25

摘要/Abstract

摘要： 对阿霉素急性肾病模型大鼠的制作方法进行了改进，并对其生化、病理及基因转录水平进行研究。结果表明，改造后的模型血浆蛋白降低，尿蛋白升高至５１２ ±９８．９ｍｇ／２４ｈ，模型典型而稳定，成活率明显提高，达９０％。电镜观察结果符合微小病变型肾病的病理变化。鼠肝白蛋白ｍＲＮＡ转录水平为对照组的２－０１倍。提示该模型的改造是成功的

关键词: 微小病变型肾病, 阿霉素肾病模型, 基因转录

Abstract: ? The nephrotic syndromed rat caused by the level of adriamycin is a good animal model and similar to the symptom of human minimal change in nephropathy. Here we report a new method causing experimental nephrotoxicity in rats by twice intravenous injection of adriamycin. Proteinuria appeared in one week after treatment. By the end of 5th. Week, proteinuria had reached the level of(512±98.9) mg/24h, and the plasma protein concentration was reduced significantly. Widespread fusion of foot processes could be found in rat glomerulus by electron microscopy. The ALB mRNA was detected by Northern and Dot blot. Albumin gene transcription was enhanced in liver of nephrotic rats and about as twice as that of the control one.

Key words: Adriamycin\ Nephropathy\ Gene transcription

鲁斌,李新民,马融. 对改造的阿霉素肾病模型的评价[J]. 实验动物科学.

Lu Bin, Li Xinmin, Ma Rong First Teaching Hospital of Tianjin Traditional Chinese Medical College 〔. Evaluation of the Modified Adriamycin Induced Nephrotic Rats[J]. laboratory animal science.