关键词: 金黄色葡萄球菌, 核酸探针, 斑点杂交

Abstract: To establish a sensitive dot blotting for detecting Staphylococcus aureus(S.aureus), a DNA fragment of 678 bps of the nuc gene was amplified from S.aureus genome DNA by Polymerase Chain Reaction(PCR) and labeled with biotin as the probe.The purified genomic DNAs of S.aureus,Streptococcus,Escherichia coli and Pasteurella were blotted onto hybridization membrane and hybridized under high stringency conditions.The Biotin\|labeled probe reacted positively with the S.aureus genomic DNAs,but not with that of the non\| S.aureus with a sensitivity of 1 pg.240 clinical samples taken randomly from laboratory mice and rats were submitted to detection by the dot blotting and previously established PCR with a positive detection rate of 2.9%(7/240) and an agreement of 100%.These data demonstrated that the established dot blotting was a quick,sensitive and specific method for detection of S.aureus in laboratory animals.

Key words: Staphylococcus aureus, Biotin\|labeled probe, Dot blotting