关键词: 布鲁杆菌, 试管凝集试验, 菌液, 抗血清

Abstract: Objective For producting bacterial liquid of cuvette agglutination reaction and antiserum against \%Brucella\% spp. of laboratory dog. Methods\ The live culture medium was washed with 0 5% phenyl hydroxide and 0 9% sodium chloride. The ideal bacterial concentration was confirmed by assimilating with standard bacterial liquid. Through infecting the antigen intravenously into Beagle dog and rabbit the antiserum was obtained, and the antiserum was characterized according to cuvette agglutination reaction. Results\ S_antiserum value of rabbits was above 1:3200, R_antiserum value was above 1:2560 Antiserum value of Beagle dogs was lower only up to 1:640 Conclusion We can obtain higher S-serum by injecting directly S-Brucella liquid to ear vein, but we can obtain higher R-antiserum when immunological agent is added to R-Brucella liquid by injecting muscle.

Key words: Brucella spp., Cuvette agglutination reaction, Bacterial liquid, Antiserum, Serum value