禽霍乱琼扩抗原和抗血清的制备

实验动物科学

禽霍乱琼扩抗原和抗血清的制备

翟新验,富强,张延钢,贾琨,卢胜明,刘钧

北京实验动物研究中心,北京实验动物研究中心,北京实验动物研究中心,北京实验动物研究中心,北京实验动物研究中心,北京市动物检疫站北京100012 ,北京100012 ,北京100012 ,北京100012 ,北京100012 ,北京100044

收稿日期:2004-12-25 修回日期:2004-12-25 出版日期:2004-12-25 发布日期:2004-12-25

Preparation of Gel Diffusion Antigen and Anti-serum of Fowl Cholera

ZHAI Xin-yan~1, FU Qiang~1, ZHANG Yan-gang~1, JIA Kun~1, LU Sheng-ming~1,LIU Jun~2

(1 Beijng Laboratory Animal Research Center, Beijing 100012,China)(2 Beijing Animal Quarantine Station, Beijing 100044,China

Received:2004-12-25 Revised:2004-12-25 Online:2004-12-25 Published:2004-12-25

摘要/Abstract

摘要： 将禽多杀性巴氏杆菌 1,3和 4型菌株 ,分别接种于加有绵羊血红素的马丁氏肉汤中进行培养 ,将培养物混合后离心 ,并漂洗沉淀物 ,制成琼扩抗原 ,与NDV ,HAAV ,APIV ,IC ,SP ,MG ,MS和IBDV等抗血清无交叉反应。将 3个血清型细菌培养物等量混合后 ,免疫 6周龄SPF鸡制备禽霍乱抗血清

关键词: 巴氏杆菌, 琼扩抗原, 禽霍乱抗血清

Abstract: Individually culturating Pasteurella multocida serotype 1, 3 and 4 were inoculated into martin broth that contains sheep hemochrome. The three bacteria culture were mixed, spinned, followed by wash the precipitate, then gel diffusion antigen was produced, which was no cross-reaction with anti-sera to NDV, HAAV, APIV, IC, SP, MG, MS, IBDV. The same mixtare of the three bacteria culture was used to immunize four-old week SPF chicks,and prepare anti-serum to fowl cholera.

Key words: Pasteurella multocida, Gel diffusion antigen, Anti-serum to fowl cholera

翟新验,富强,张延钢,贾琨,卢胜明,刘钧. 禽霍乱琼扩抗原和抗血清的制备[J]. 实验动物科学.

ZHAI Xin-yan~1, FU Qiang~1, ZHANG Yan-gang~1, JIA Kun~1, LU Sheng-ming~1,LIU Jun~2. Preparation of Gel Diffusion Antigen and Anti-serum of Fowl Cholera[J]. laboratory animal science.

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