关键词: 裸小鼠, 层流柜

Abstract: Objective To summarize the experience of the reproduction and application of the experimental animal nude mice for 8 years in our laboratory. Methods Randomly drawing out 20 litters of BALB/c nude mice and 5 maternal nude mice.analyze t heir generational state. Results Averagely, one BALB/c nude mou se gives birth to 9.35 mice every time. Female nu/+ and male nu/nu crossbreeding, 55.0 per cent of offspring are nu/ nu homozygous mice,and 97.0 per cent of offspring survives after ablactation.From the first-born to forth-born,the fecundity is steady, the survival rate for 1 year is 100 per cent,and the longe st survival period is 752 days.Microbiological detection couldn’t find any pathogen or parasite whic h should be controlled. Conclusion Under the condition of 100?000 grade air filtrating and in 10?000 grade lamina r flowing cabinet,with strict aseptic manipulation,we bred qualified specific pathogen free(SPF) nude mice.

Key words: Nude mice, Laminar flowing cabinet, Reproducti on